Amersham WB automated Western blot

One Bench at a Time

Amersham WB automated Western blot

Fully integrated system for SDS-PAGE and Western blotting of proteins based on fluorescence detection.

  • Integrated Western blotting system with standardized protocols in every step minimizes assay variability.
  • Dedicated consumables and reagents ensure high quality and reproducibility.
  • Optimized prelabeling of samples with Cy5 NHS chemistry for proven performance, eliminating the need for gel staining.
  • Reliable normalization by multiplexing target and control fluorescent signals on the same blot.
  • Western blot workflow achieved in less than 4 h ensures fast, conclusive data.
  • Smart control and evaluation software gives you complete control.

The Amersham WB system consists of the Amersham WB analyzer, Amersham WB software, and dedicated consumables for electrophoresis and Western blotting.

Amersham WB Analyzer

The Amersham WB analyzer is divided into two instrument units—one for electrophoresis and scanning and one for the full Western blot workflow including transfer, probing, and drying. The electrophoresis & scanning unit is used to separate SDS-treated sample proteins in a polyacrylamide gel. It is also used to scan gels after electrophoresis and scan polyvinylidene (PVDF) cards after probing and drying in a Western experiment.

The Western unit is used to:

    • (1) transfer separated proteins in the gel to the PVDF membrane;
    • (2) probe proteins on the membrane with primary antibodies and secondary antibodies conjugated to a CyDye;
    • (3) dry the PVDF cards (before scanning in the electrophoresis & scanning unit).

The Amersham WB system provides standardized, quantitative, and reproducible analysis of proteins in your samples in a significantly shorter time compared with traditional workflows/procedures.

Learn more at GE Healthcare

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Upcoming Event

  • Tosoh Hour

    14:00-15:30
    12/14/2017
    1. HPLC Products 2. Business Development and Venture Investment Our newest sponsor Tosoh will be here on December 14th. Amongst Tosoh's interests is the Separations and Purification Division.  Their scientist, Philip Hoang, will be here and would be happy to meet with you to discuss your purification challenges. He will be joined by Toshifumi Mogami and Kenichi Haga from Tosoh's Venture and Business Development Units. Tosoh is most interested in companies working in diagnostics and research tools.  Please contact Linda Eng if you'd like to meet with them. Toshifumi Mogami, PhD, is Vice President of Business Development & Venture Investment from Tosoh USA, Inc., the venture capital arm of Tosoh Corporation. Toshifumi has a background in biophysics and bioengineering. Kenichi Haga is Senior Vice President of Business Development and Venture Investment from Tosoh USA, Inc. and his background is in organic chemistry. Philip Hoang is an Account Manager of HPLC Columns for the West Coast. He is responsible for sales and support of HPLC/UHPLC columns in California, Oregon and Washington state. Philip received his bachelor’s degree in biology from California State University, Long Beach. He has five years of technical experience in the HPLC and UHPLC field, where his work focused on the analysis of mAbs and ADCs.

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